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MicroVue Serum PYD EIA
貨號：8019
品牌：Quidel
規(guī)格：96 Test
目錄價：詢價
商品詳情 相關(guān)下載

For Research Use Only in the United States.
Not for use in diagnostic procedures.

MicroVue Serum PYD provides a quantitative measure of the excretion of pyridinoline cross-link (Pyd) in serum. Pyridinoline cross-links are an indicator of type I collagen resorption, especially bone collagen.

In humans, the total pool of urinary Pyd is approximately 45% free while the remaining fraction is bound to oligopeptides ranging from small linear peptides to very large cross-linked structures in excess of 10,000 Da. The proportion of free to total cross-links appears to be constant in healthy individuals and those with metabolic bone diseases or arthritis conditions, thus providing the rationale for measuring free Pyd.

Performance Characteristics

Method: Competitive
Analyte: Pyridinoline
Specimen: Serum
Specimen Volume: 25 μL post-filtration
LOD*: 0.4 nmol/L
LLOQ*: 0.75 nmol/L
HLOQ*: 12 nmol/L
Precision CVs within-run: 6-15%
Precision CVs between-run: 9-12%
Incubation Time: 20-26 Hours
Cross-reactivity: Free PYD 100% Free DPD 0% PYD/DPD peptides < 2.5% L-arginine 0.0004% (see Package Insert)

*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification

About the Assay

Format

96 well microtiter plate, with reagents sufficient to test 40 samples in duplicate

Antibody Specificity

Free pyridinoline cross-links, some cross-reactivity to glutamine and arginine (0.5 nmol/L signal)

Species Reactivity

Baboon, Cat, Cow, Dog, Guinea Pig, Horse, Human, Rhesus Macaque, Mouse, Rat 
No reactivity detected with Rabbit

Assay Steps

Spin serum in Spinfilters (included) for 30 minutes
Add 50 μL Reagent 1 to plate
Add 25 μL serum, diluted Standards, diluted Controls
Add 75 μL cold Pyridinoline Antibody
Incubate overnight at 2°C to 8°C
Wash 3 times with 1x Wash Buffer
Add 150 μL Enzyme Conjugate
Incubate 1 hour at room temperature
Wash 3 times with 1x Wash Buffer
Add 150 μL working Substrate Solution
Incubate 40 +/– 5 minutes at room temperature
Add 100 μL Stop Solution
Read at 405 nm
Data Reduction: 4 Parameter